SOP · BIOSECURITY
Facility Biosecurity Zoning & Entry
ActiveBiosecurityRev D
Purpose
To control movement between biosecurity zones within HIVE-13 facilities so that pathogens, invasive specimens, and contaminants never cross from a dirty zone into a clean one. The procedure guarantees that entry follows a strict clean-to-dirty gradient, that no person crosses a zone boundary without the required decontamination, and that the most restricted zones — live invasive containment and clean broodstock — are never compromised by an uncontrolled crossing.
Scope
All zoned areas within HIVE-13 facilities.
In scope:
- Zone classification and gradient definition
- Entry authorization and decontamination at boundaries
- Directional movement discipline
- Cleared egress and PPE disposal
Out of scope:
- Live invasive specimen containment within the dirty zone, which follows SOP Invasive Hornet Intercept & Quarantine
- Seized-goods storage, which follows SOP Seized-Goods Handling
Definitions
Clean-to-Dirty Gradient
The directional rule that movement proceeds from cleaner to dirtier zones, never the reverse without decontamination.
Decontamination Boundary
A controlled threshold where PPE change and decontamination occur before crossing into a different zone.
Restricted Zone
The highest-control area — live invasive containment or clean broodstock — entered only by authorized personnel.
Uncontrolled Crossing
Any zone transition without the required decontamination, treated as a containment breach.
Responsibilities
Biosecurity Lead (Owner)
Owns zoning; defines the gradient and authorizes restricted-zone access.
Zone Entry Officer
Verifies authorization and decontamination at each boundary and logs every crossing.
HIVE-13 Science Board
Sets the zone classifications and decontamination requirements.
MELISSA
Logs boundary crossings against credentials and flags any uncontrolled crossing as a breach.
Procedure
Classification
- Classify every facility area by biosecurity zone and post the clean-to-dirty gradient.
- Identify restricted zones requiring authorized access only.
- Define the decontamination requirement at each zone boundary.
Entry
- Verify authorization for the destination zone before crossing any boundary.
- Move only along the clean-to-dirty gradient; never cross back without decontamination.
- Complete the required PPE change and decontamination at each boundary.
Egress
- Decontaminate and change PPE before egressing to a cleaner zone.
- Dispose of used PPE in the dirty-zone waste stream only.
- Log every crossing; treat any uncontrolled crossing as a containment breach and report it.
PPE & Controls
Zone-specific gowning issued at each boundarySingle-use gloves and overshoesRespirator in live invasive containment zonesFootbath and decontamination station at each boundary
Records Generated
- Zone classification map (retained until superseded)
- Boundary-crossing log with credentials (retained 3 years)
- Decontamination compliance record
- Uncontrolled-crossing breach reports
References
- SOP: Invasive Hornet Intercept & Quarantine
- SOP: Seized-Goods Handling
- SOP: MELISSA Alarm Triage & Escalation
- HIVE-13 Decontamination Standard
Revision History
| Rev | Date | Note |
|---|---|---|
| A | 2089-02-28 | Initial issue; zone classification and gradient established. |
| B | 2089-10-15 | Added decontamination boundaries and directional movement rule. |
| C | 2090-08-30 | Added restricted-zone authorization and PPE-disposal discipline. |
| D | 2091-01-22 | MELISSA crossing logging and uncontrolled-crossing breach flagging formalized. |